Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: The cuproptosis blocker SS-31 ameliorated airway responsiveness and airway inflammation in chronic asthma model mice (n = 10 mice per group). ( A ) Ers, Rrs and Rn in different groups challenged with aerosol Mch (mg/ml). ( B ) Representative images of HE-stained lung tissue. C. Cell classification (eosinophils, lymphocytes, and neutrophils) and counts in BALF. D. The levels of the inflammatory factors IL-4, TGFβ1 and IL-1β in the BALF. The data are shown as the means ± SEMs, n = 10. One-way analysis of variance (ANOVA) and the Student–Newman–Keuls (SNK) method were used, and P < 0.05 was considered to indicate statistical significance. *p < 0.05 for the OVA group vs. the control group. # p < 0.05 for the SS-31 + OVA group vs. the PBS + OVA group. Above experiment was repeated three times.

Article Snippet: Cytokine levels (interleukin-4 (IL)-4, TGFβ1 and IL-1β) (Multi Sciences Biotech Co., Ltd) in the BALF of the mice were measured via individual ELISA kits according to the manufacturers’ instructions.

Techniques: Aerosol, Staining, Control

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: Cuproptosis was increased in BEAS-2B cells incubated with rhIL-4 and TGFβ1 and the addition of SS-31 reversed the above process. ( A ) Quantification of the effects of different concentrations of SS-31 on the cell viability of BEAS-2B cells. ( B ) Cu 2+ levels in the supernatant of BEAS-2B cells. ( C ) Cu 2+ levels in the cytoplasm of BEAS-2B cells. D. Protein expression of cuproptosis-related genes (FDX-1, LIAS, and DLAT) in BEAS-2B cells. ( D ) Quantification of the protein expression levels of cuproptosis-related genes in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the SS-31 + rhIL-4 + TGFβ1 group vs. the PBS + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Article Snippet: Cytokine levels (interleukin-4 (IL)-4, TGFβ1 and IL-1β) (Multi Sciences Biotech Co., Ltd) in the BALF of the mice were measured via individual ELISA kits according to the manufacturers’ instructions.

Techniques: Incubation, Expressing

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: Silencing FDX-1 attenuated cell remodeling in BEAS-2B cells incubated with rhIL-4 and TGFβ1. ( A , B ) Quantification of FDX-1 protein expression in BEAS-2B cells transfected with different siRNAs. ( C ) Cell morphology in different groups was observed under a light microscope. ( D , E ) Quantification of the effect of FDX-1 knockdown on cell migratory function. F. Effects of FDX-1 knockdown on the protein expression of EMT-related MMP-2, MMP-9, E-cadherin, N-cadherin, vimentin and smooth muscle-related α-SMA in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the Scr + rhIL-4 + TGFβ1 group vs. the Si + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Article Snippet: Cytokine levels (interleukin-4 (IL)-4, TGFβ1 and IL-1β) (Multi Sciences Biotech Co., Ltd) in the BALF of the mice were measured via individual ELISA kits according to the manufacturers’ instructions.

Techniques: Incubation, Expressing, Transfection, Light Microscopy, Knockdown

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: NAT10-Mediated ac4C-Modification Exacerbates Ferroptosis by Stabilizing HMOX1 in Deep Vein Thrombosis

doi: 10.1161/ATVBAHA.125.323986

Figure Lengend Snippet: Knockout NAT10 ( N -acetyltransferase 10) represses HMOX1 (heme oxygenase 1) to reduce the formation of deep vein thrombosis (DVT) in vivo. A and B , Thrombus weights and length are measured in the different treatment groups (n=10 mice per group). C and D , Representative images of thrombi in NAT10 fl /fl Cdh5-Cre + (NAT10 knockdown) mice were detected by hematoxylin and eosin staining (magnification, ×100) and vascular ultrasound. Scale bars=200 µm. E through G , Relative expression levels of ferrous ions (Fe 2+ ), malondialdehyde (MDA), and glutathione (GSH) in different treatment groups (n=10 mice per group). H , Expressions of HMOX1 and GPX4 (glutathione peroxidase 4) are determined by Western blotting in different treatment groups (n=6 mice per group). I , The expression of HMOX1 is determined by quantitative real-time polymerase chain reaction (qRT-PCR) in different treatment groups (n=10 mice per group). J through M , Expression of ET-1 (endothelin-1), eNOS (endothelial NO synthase), TNF-α (tumor necrosis factor-α), or TGF-β1 (transforming growth factor beta 1) are determined by ELISA in the plasma of each treatment group (n=10 mice per group). Results are expressed as mean±SEM. Statistical analysis was performed by 1-way ANOVA with Tukey post hoc tests ( A, B, E through L ) and Brown-Forsythe ANOVA with Games-Howell post hoc tests ( M ). CoPP indicates cobalt protoporphyrin IX.

Article Snippet: The protein expressions of eNOS (endothelial NO synthase), ET-1 (endothelin-1), TNF-α (tumor necrosis factor-α), and TGF-β1 (transforming growth factor beta 1) were detected following the manufacturer’s instructions with ELISA Kits (Cusabio, Wuhan, China; Multi Sciences, Hangzhou, China).

Techniques: Knock-Out, In Vivo, Knockdown, Staining, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Clinical Proteomics